Work-Study Student Handbook

Chen Xu

Copyright © 2003 2004 2005 2006 Brandeis EM Lab.

Thank you for becoming a part of Brandeis Electron Microscopy Facility. Your contribution is extremely valuable.

This handbook contains instructions for many of the work-study student jobs, as well as general information about the how the lab operate.

This document is a work in progress, and is not complete. Sections that are known to be incomplete are indicated with a * in their names.

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Table of Contents
Preface
Welcome!
* Directory
Overview List of Duties
Acknowledgments
1. Darkroom Maintenace
1.1. Darkroom Maintenace Schedule
1.2. D-19 Developer
1.3. Fixer
1.4. General Procedures
1.5. Notes on Specific Darkrooms:
1.6. Developing Film
1.6.1. Unloading/Loading Film--CM12, Philip 420 and Morgagni 268
1.6.2. Film Developing Instructions
2. Laboratory Maintenace
2.1. Lab Maintenace Schedule
2.2. General Procedures
3. Lab Supplies
3.1. Restocking Lab Supplies
3.1.1. Items which can be purchased in the Rosenstiel stockroom
3.1.2. Items that will need to be ordered from an outside supplier
4. Equipment Maintenace
4.1. Equipment Maintenace Schedule
4.2. IN-HOUSE MAINTENANCE INFORMATION
4.3. Nitrogen Manifolds
4.4. Monthly Service Checks for the Equipment in the Laboratory
5. Electron Gun
5.1. Cleaning the Electron Gun for Morgagni
6. Edwards Evaporator
6.1. Cleaning the Edwards Bell Jar
6.2. Edwards 306 Vacuum Operating Instructions
6.2.1. Start-Up from Either Cold Status or Partially Warm Status:
6.2.2. Turning the Instrument On:
6.2.3. Start-Up from Partially Warm Status
6.2.4. Vacuum Evaporation of Metals:
6.2.5. Venting the Chamber to Remove Your Samples and Leaving the Instrument in Stand-by for the Next User:
6.2.6. To Shut the Instrument Off:
7. Grid Perparation Methods
7.1. Preparation of Carbon Coated Mica for Making "Carbon Only" Grids
7.2. Carbon Only Grids
7.3. Preparation of Formvar and Holey-Formvar Stock and Working Solutions:
7.4. Preparation of Holey Formvar Working Solution
7.5. Preparation of Formvar and Holey-Formvar Coated Grids
7.6. Making Formvar Grids in High Humidity
7.7. Carbon Coating Formvar and Holey Formvar Grids
7.8. Holey Triafol Grids:
8. Making stains for Electron Microscopy
8.1. Uranyl Acetate Prep and Packaging:
8.1.1. Preparing 4% Uranyl Acetate Stock Solution:
8.1.2. Preparing 1% and 2% Uranyl Acetate Solutions:
8.1.3. Preparing 1% and 2% Urnayl Acetate for use:
8.2. Rotary Shadow Instructions
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